RNA-Seq - Compare expression between sample types
- Due Apr 19, 2019 at 11:59pm
- Points 26
- Questions 10
- Time Limit None
- Allowed Attempts 3
Instructions
The goal of this quiz is to help you check your understanding of the RNA-Seq data analysis code presented in class.
Note: Re-download the file to make sure you have the latest (best) version.
The following questions below are designed to highlight the most important elements of the script.
Key ideas:
- Negative log2 fold-change indicates down-regulation
- Positive log2 fold-change indicates up-regulation
- Use adjusted p-values and fold-change cutoffs to decide whether a gene is differentially expressed. These cutoffs are somewhat arbitrary and often depend on the goals of the experiment.
Tip: Write analysis code in a flexible way so that you can change the cutoffs easily and re-run your code if required.
Tip: First quickly read the questions. Then use the script to answer them.
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